Construction of recombinant plasmid expressing S1 gene of new type of reovirus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 361-363, 2011.
Article
em Zh
| WPRIM
| ID: wpr-246239
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the recombinant plasmid containing S1 gene of new type of reovirus, and to study the expression of protein sigma1 in Vero cells.</p><p><b>METHODS</b>The recombinant plasmid, named pC-S, was constructed by cloning S1 gene into vector pCAGGS/MCS. Then Vero cells were transfected with pC-S and collected at 24, 48, 72 h post transfection followed by SDS-PAGE and Western-Blot assay.</p><p><b>RESULTS</b>Results both SDS-PAGE and Western-Blot assay indicated that sigma1 protein could be expressed well and the highest expression level was 72 h post transfection.</p><p><b>CONCLUSIONS</b>Sigma1 protein could be expressed well in Vero cells by transfected with recombinant plasmid containing S1 gene, and could give some implications for subsequent research on virus-host interactions.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plasmídeos
/
Reoviridae
/
Proteínas Virais
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Células Vero
/
Proteínas Recombinantes
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Expressão Gênica
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Chlorocebus aethiops
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Genética
/
Metabolismo
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2011
Tipo de documento:
Article