Role of insulin-like growth factor II receptor in transdifferentiation of free silica-induced primary rat lung fibroblasts / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 979-985, 2013.
Article
em En
| WPRIM
| ID: wpr-247101
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts.</p><p><b>METHODS</b>Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of α-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.</p><p><b>RESULTS</b>The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.</p><p><b>CONCLUSION</b>The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.</p>
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Fisiologia
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RNA Mensageiro
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Sequência de Bases
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Diferenciação Celular
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Células Cultivadas
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Receptor IGF Tipo 2
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Ratos Sprague-Dawley
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Primers do DNA
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Dióxido de Silício
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2013
Tipo de documento:
Article