Plasticity of regulation of mannitol phosphotransferase system operon by CRP-cAMP complex in Vibrio cholerae / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 831-840, 2013.
Article
em En
| WPRIM
| ID: wpr-247126
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>The complex of the cyclic AMP receptor protein (CRP) and cAMP is an important transcriptional regulator of numerous genes in prokaryotes. The transport of mannitol through the phosphotransferase systems (PTS) is regulated by the CRP-cAMP complex. The aim of the study is to investigate how the CRP-cAMP complex acting on the mannitol PTS operon mtl of the Vibrio cholerae El Tor biotype.</p><p><b>METHODS</b>The crp mutant strain was generated by homologous recombination to assess the need of CRP to activate the mannitol PTS operon of V. cholerae El Tor. Electrophoretic mobility shift assays (EMSA) and the reporter plasmid pBBRlux were used to confirm the role that the CRP-cAMP complex playing on the mannitol PTS operon mtl.</p><p><b>RESULTS</b>In this study, we confirmed that CRP is strictly needed for the activation of the mtl operon. We further experimentally identified five CRP binding sites within the promoter region upstream of the mannitol PTS operon mtl of the Vibrio cholerae El Tor biotype and found that these sites display different affinities for CRP and provide different contributions to the activation of the operon.</p><p><b>CONCLUSION</b>The five binding sites collectively confer the strong activation of mannitol transfer by CRP in V. cholerae, indicating an elaborate and subtle CRP activation mechanism.</p>
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Base de dados:
WPRIM
Assunto principal:
Óperon
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Fosfotransferases
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Proteínas de Bactérias
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Vibrio cholerae
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Dados de Sequência Molecular
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Sequência de Bases
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Regulação Bacteriana da Expressão Gênica
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AMP Cíclico
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Proteína Receptora de AMP Cíclico
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Genética
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2013
Tipo de documento:
Article