Sequence analysis and expression study of BRI gene in two lung adenocarcinoma cell lines with different metastatic potential / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 292-296, 2003.
Article
em Zh
| WPRIM
| ID: wpr-248437
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the sequence of amyloid fibrils (BRI) gene and its expression in two lung adenocarcinoma cell lines AGZY83-a and Anip973 with the same tumor origin but different metastatic potential.</p><p><b>METHODS</b>DNA sequencing, sequential G banding fluorescence in situ hybridization (FISH) and Northern blot were used to analyze the sequence and expression of BRI gene was in two lung adenocarcinoma cell lines with different metastatic potential.</p><p><b>RESULTS</b>The expression of BRI gene was up-regulated in the highly metastatic cell line Anip973 and was down-regulated in the low metastatic cell line AGZY83-a from which the Anip973 was derived. FISH results disclosed that in the two cell lines, the same rearrangements existed in the chromosome region where BRI gene was located, but in Anip973, amplification took place in the chromosome region where BRI gene was located. DNA sequencing results showed different mutations in the 5' untranslated region of BRI gene in the two cell lines.</p><p><b>CONCLUSION</b>The above results revealed that there was no relation between BRI gene differential expression and rearrangements of chromosome. The amplification of the chromosome region where BRI gene was located and the different mutations in the 5' untranslated region of BRI gene probably contributed to the differential expression.</p>
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Cromossomos Humanos Par 13
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Glicoproteínas de Membrana
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Adenocarcinoma
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Regulação Neoplásica da Expressão Gênica
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Northern Blotting
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Análise de Sequência de DNA
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Hibridização in Situ Fluorescente
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Linhagem Celular Tumoral
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2003
Tipo de documento:
Article