Molecular characterization of hantavirus Zhejiang isolate ZT10 strain from M. fartis / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 2-5, 2008.
Article
em Zh
| WPRIM
| ID: wpr-248740
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To learn about the complete genomic sequence of the Seoul virus strain ZT10 isolated from M. fartis.</p><p><b>METHODS</b>The total RNA was extracted from the infected Vero E6 cells and amplified by RT-PCR. The purified PCR products were cloned into T-vector and sequenced.</p><p><b>RESULTS</b>The results demonstrated that the complete genome of ZT10 was comprised of L(6530), M(3651) and S(1753) segments which encoded 2151-1133 and 429 amino acids respectively.</p><p><b>CONCLUSION</b>Analysis of sequence revealed that the ZT10 belonged to Seoul virus. The nucleotide sequence identity of the M gene with Seoul virus was 84.0%-96.3%. The identity with Hantan vrisu (Prospect Hill virus, Tula virus) isolated from M. fartis was 57.5%-60.9%. The sequence identity of the S gene with Seoul virus was 87.9%-96.0% at nucleotide level and 96.9%-97.9% at amino acid level.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Filogenia
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DNA Viral
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Linhagem Celular
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Reação em Cadeia da Polimerase
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Alinhamento de Sequência
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Classificação
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Clonagem Molecular
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Orthohantavírus
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Análise de Sequência de DNA
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DNA Complementar
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Article