XBP-1 interacts with estrogen receptor alpha (ERalpha) / 生物工程学报
Chinese Journal of Biotechnology
; (12): 332-336, 2004.
Article
em Zh
| WPRIM
| ID: wpr-249987
Biblioteca responsável:
WPRO
ABSTRACT
Estrogen receptor alpha (ERalpha) has been a primary target of treatment as well as a prognostic indicator for breast cancer. The level of human X-box binding protein 1 (XBP-1) mRNA was related with that of ERalpha in breast tumors and was over-expressed in some breast tumors. These previous studies suggested that XBP-1 may interact with ERalpha. XBP-1 has two isoforms, XBP-1S and XBP-1U, as the result of unique splicing. GST pull-down assay showed that both XBP-1S and XBP-1U bound to ERalpha in vitro. The binding of XBP-1S to ERalpha was stronger than that of XBP-1U to ERalpha. Co-immunoprecipitation revealed that the binding was in a ligand-independent manner. XBP-1S and XBP-1U interacted with the region of ERalpha that contains a DNA-binding domain. The ERalpha-interacting regions on XBP-1S and XBP-1U have been mapped to two regions, the N-terminal basic region leucine zipper domain (bzip) and the C-terminal activation domain. These findings suggest that XBP-1S and XBP-1U may participate in ERalpha signaling pathway through the mediation of ERalpha.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fisiologia
/
Fatores de Transcrição
/
Neoplasias da Mama
/
RNA Mensageiro
/
Transdução de Sinais
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Linhagem Celular Tumoral
/
Receptor alfa de Estrogênio
/
Proteínas de Ligação a DNA
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Domínios e Motivos de Interação entre Proteínas
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Proteína 1 de Ligação a X-Box
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2004
Tipo de documento:
Article