Enhancements in ethanol tolerance of a self-flocculating yeast by calcium ion through decrease in plasmalemma permeability / 生物工程学报
Chinese Journal of Biotechnology
; (12): 715-719, 2003.
Article
em En
| WPRIM
| ID: wpr-250002
Biblioteca responsável:
WPRO
ABSTRACT
Ca2+ at 1.64 mmol/L markedly increased ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae. After 9 h of exposure to 20% (V/V) ethanol at 30 degrees C , no viability remained for the control whereas 50.0% remained for the cells both grown and incubated with ethanol in Ca2+ -added medium. Furthermore, when subjected to 15% (V/V) ethanol at 30 degrees C, the equilibrium nucleotide concentration and plasma membrane permeability coefficient (P' ) of the cells both grown and incubated with ethanol in Ca2+ -added medium accounted for only 50.0% and 29.3% those of the control respectively, indicating that adding Ca2+ can markedly reduce plasma membrane permeability of yeast cells under ethanol stress as compared with the control. Meanwhile, high viability levels acquired by the addition of Ca2+ exactly corresponded to the striking decreases in extracellular nucleotide concentration and P' achieved with identical approach. Therefore, the enhancing effect of Ca2+ on ethanol tolerance of this strain is closely related to its ability to decrease plasma membrane permeability of yeast cells subjected to ethanol stress.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Saccharomyces cerevisiae
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Schizosaccharomyces
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Temperatura
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Membrana Celular
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Permeabilidade da Membrana Celular
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Cálcio
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Etanol
/
Metabolismo
Idioma:
En
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2003
Tipo de documento:
Article