Expression and amplification of steroid receptor coactivator-3 gene in colorectal carcinoma and its clinicopathological significance / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 67-70, 2005.
Article
em Zh
| WPRIM
| ID: wpr-252462
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and amplification of steroid receptor coactivator- 3(SRC- 3) gene in colorectal carcinoma (CRC) and its clinicopathological significance.</p><p><b>METHODS</b>Immunohistochemistry and fluorescence in situ hybridization (FISH) were used to detect the expression and amplification of SRC- 3 gene in CRC, and its association with patient's clinical pathological features was analyzed.</p><p><b>RESULTS</b>A total of 60 patients with CRC were studied. SAR- 3 proteins were overexpressed in 23 cases (38% ). There was a significant association between SAR- 3 overexpression and neoplasm staging (P< 0.01). SRC- 3 protein was overexpressed in 62% of patients with Dukes C or D stage, whereas SRC- 3 protein was normally expressed in 74% of patients with Dukes A or B stage. As for FISH study, 47 cases were informative. High- level amplification of SRC- 3 gene was detected in 6 cases(13% ) and all showed overexpression of SRC- 3 protein. Low- level amplification of SRC- 3 was observed in 9 cases (19% ). Overexpression of SRC- 3 was detected in 6 cases. The remaining 9 of 32 patients(28% ) without amplification of SRC- 3 gene were observed with overexpression of SRC- 3 protein. In addition, 91% patients with CRC were found overexpression of SRC- 3 as well as overexpression of P53.</p><p><b>CONCLUSION</b>The abnormal expression of SRC- 3 gene might impact on the function of P53 and development of CRC. There might exist some unknown mechanisms other than gene amplification of SRC- 3 to regulate its encoded protein expression in CRC.</p>
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Neoplasias Colorretais
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Biomarcadores Tumorais
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Expressão Gênica
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Transativadores
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Histona Acetiltransferases
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Coativador 3 de Receptor Nuclear
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Genética
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Estadiamento de Neoplasias
Limite:
Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2005
Tipo de documento:
Article