Dynamics of calcium in the hippocampal neuronal culture model of epilepsy / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 200-203, 2007.
Article
em Zh
| WPRIM
| ID: wpr-253446
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To observe the intracellular calcium ion spatio-temporal and dynamic changes in the hippocampal neuronal culture model of epilepsy induced by low magnesium ion medium, and to explore the relationship between calcium ion and epilepsy.</p><p><b>METHODS</b>Applying both laser scanning confocal microscope and patch clamp to timely observe the changes of [Ca2+]i and electrophysiological in the hippocampal neuronal culture model of epilepsy, and the influence of NMDA receptor-gated channels retarder and non-NMDA receptor-gated channels retarder.</p><p><b>RESULTS</b>After the hippocampal nerve cell broken into epileptiform discharges, [(Ca2+]i rapidly ascended to (620 +/- 70) nmol/L, NMIDA acceptor retarder (MK-801, 10 micromol/L) and non-NMDA acceptor retarder (NBQX, 10 micromol/L) reduced [Ca2+]i ascendance. Recovery of the elevated [Ca2+]i was obviously delay, after 90 min and 150 min epileptiform discharges, it took (114.8 +/- 5.2) min and (135.0 +/- 22.7) min (P < 0.05) respectively.</p><p><b>CONCLUSION</b>In vitro status epilepticus causes sustained elevation of intracellular calcium levels in hippocampal neurons</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Estado Epiléptico
/
Células Cultivadas
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Cálcio
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Ratos Sprague-Dawley
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Biologia Celular
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Hipocampo
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Animais Recém-Nascidos
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Metabolismo
/
Neurônios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2007
Tipo de documento:
Article