Effect of elastic stress on expression of corebinding factor a 1 mRNA in human periodontal ligament cells in vitro / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 374-376, 2003.
Article
em Zh
| WPRIM
| ID: wpr-253693
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the osteoblast-like characteristics of human periodontal ligament cells affected by elastic stress in vitro, and the role of corebinding factor a 1 (cbfa1) in alveolar bone formation during orthodontic tooth movement.</p><p><b>METHODS</b>Rat dig-labeled cbfa1 cDNA probe was prepared from SD rat osteoblasts cultured in vitro. Human periodontal ligament cells were cultured on the elastic bottom plate and stimulated by elastic stress using mechanical loading system for cultured cells in vitro. The expression of cbfa1 mRNA was detected by in situ hybridization method.</p><p><b>RESULTS</b>Cbfa1 mRNA express in human periodontal ligament cells stimulated by elastic stress and did not express in normal human periodontal ligament cells.</p><p><b>CONCLUSION</b>It is suggested that elastic stress plays a role in the differentiation process from human periodontal ligament cells to osteoblast-like cells. Cbfa1 is a transcription factor in alveolar bone remodeling during orthodontic tooth movement.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Ligamento Periodontal
/
Fatores de Transcrição
/
RNA Mensageiro
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Células Cultivadas
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Biologia Celular
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Proteínas de Ligação a DNA
/
Elasticidade
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Subunidades alfa de Fatores de Ligação ao Core
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Genética
/
Metabolismo
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2003
Tipo de documento:
Article