Quantitative detection of hepatitis B virus covalently closed circular DNA in sera of chronic hepatitis B patients with a newly established assay / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 225-227, 2008.
Article
em Zh
| WPRIM
| ID: wpr-254096
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To quantitatively detect hepatitis B virus covalently closed circular DNA (HBV cccDNA) in sera of chronic hepatitis B patients with a newly established assay.</p><p><b>METHODS</b>Primers and probe were designed in highly conservative region of HBV DNA. DNA was extracted from 175 sera samples of chronic hepatitis B patients, and was treated with plasmid-Safe-ATP-dependent Dnase(PSAD) to eliminate the relaxed circular DNA (rcDNA). The products were amplified by real-time PCR with primers spanning.</p><p><b>RESULTS</b>The detection rate of serum HBV cccDNA was found to correlate directly with serum HBV DNA loading. HBeAg positive chronic hepatitis B patients had higher serum HBV cccDNA levels than HBeAg negative chronic hepatitis B patients.</p><p><b>CONCLUSION</b>The method is good because of the high specificity. It can be used for detection of HBV cccDNA. DNA;</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Virologia
/
Sangue
/
DNA Circular
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DNA Viral
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Vírus da Hepatite B
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Reação em Cadeia da Polimerase
/
Primers do DNA
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Hepatite B Crônica
/
Genética
/
Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
/
Adult
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Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Article