Detection of HBV DNA among HBsAg negative children after prevention for mother-to-infant transmission / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Detection HBV DNA among HBsAg negative children who have been vaccined at birth, in order to improve the evaluation of the indicator for HBV DNA infection.</p><p><b>METHODS</b>Selection HBsAg negative children who have been vaccined at birth and then detection HBV DNA from sera using QIAamp Viral DNA Mini Kit, HBV DNA s region was obtained by nested PCR and sequencing.</p><p><b>RESULTS</b>12 of the 140 children were HBV DNA detected were positive and the infectious rate was 8.6% . No mutant of the 12 HBV DNA in "a" determinant.</p><p><b>CONCLUSION</b>To evaluate the effection of the prevention of HBV mother-to-child transmission, the standard method should be established. The detection of HBV DNA should be included in the future.</p>