Quantitative analysis of circulating DNA in serum of cancer patients / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 606-608, 2004.
Article
em Zh
| WPRIM
| ID: wpr-254275
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for ng quantitation of circulating DNA in serum and explore the value in the diagnosis of cancer.</p><p><b>METHODS</b>Serum DNA was extracted by commercial "genomic DNA extraction kit" and detected by fluorescent dye (SYBR green I) staining. Loss of heterozygosity (LOH) at BRCA1 (D17S579, D17S855) and p53 (TP53, D17S786) in serum DNA was analyzed by PCR-based method.</p><p><b>RESULTS</b>SYBR green I dot staining could detect DNA as low as 2 ng. Using this method, we detected serum samples from 483 patients with various types of cancer and 150 healthy individuals. The mean DNA concentration in the normal controls was 22.2 +/- 13.4 ng/ml, while that in cancer patients was 81.3 +/- 98.3 ng/ml (P < 0.001). In 33 ovarian cancer patients with increased DNA level, 27(81.8%) displayed LOH in at least one of the four loci analyzed.</p><p><b>CONCLUSION</b>Circulating DNA in serum may become additional tumor marker for the diagnosis of cancer.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Sangue
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DNA de Neoplasias
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Biomarcadores Tumorais
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Genes p53
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Proteína Supressora de Tumor p53
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Repetições de Microssatélites
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Proteína BRCA1
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Genes BRCA1
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Perda de Heterozigosidade
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Genética
Limite:
Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2004
Tipo de documento:
Article