Effect of overexpression of Smad7 gene on cell proliferation / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 521-524, 2004.
Article
em Zh
| WPRIM
| ID: wpr-254311
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of overexpression of Smad7 gene on cell proliferation in human bronchial epithelial cell lines.</p><p><b>METHODS</b>Human bronchial epithelial cell lines, BEP2D and BERP35T2 cells, were cotransfected with the mammalian expression vectors PCISmad7.neo and pMyc-SEAP, the latter was ac-myc cis-acting enhancer element fused with alkaline phosphatase (SEAP) reporter gene. Expression of c-myc, p15 and p21 mRNA was detected by RT-PCR before and after stable transfection of Smad7 into BEP2D and BERP35T2 cells in order to study the regulation of TGF-beta-mediated growth inhibition.</p><p><b>RESULTS</b>After BEP2D and BERP35T2 cells transfected with Smad7, the transcriptional activity of c-myc was significantly increased. Smad7 overexpressing cells showed upregulation of c-myc expression and downregulation of p15 and p21 expression, which contributed to the loss of TGF-beta responses in these cells.</p><p><b>CONCLUSION</b>Overexpression of Smad7 may facilitate cell proliferation by antagonizing TGF-beta-mediated antiproliferative gene responses.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Brônquios
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Transfecção
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Transdução de Sinais
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Transformação Celular Neoplásica
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Células Cultivadas
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Proteínas Proto-Oncogênicas c-myc
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Fator de Crescimento Transformador beta
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Biologia Celular
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Proliferação de Células
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Células Epiteliais
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2004
Tipo de documento:
Article