Experimental study on induction of the normal human melanocytes apoptosis in vitro by cycloheximide and TNF-alpha / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 302-305, 2005.
Article
em Zh
| WPRIM
| ID: wpr-255047
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of cycloheximide and TNF-alpha on melanocyte (MC).</p><p><b>METHODS</b>Melanocyte apoptosis was studied with MTT, transmitting electron microscopy and fluorescence labeling of alive cells.</p><p><b>RESULTS</b>We added TNF-alpha and cycloheximide in melanocytes, and the typical apoptosis appeared 24 hours later, with chromatin condensation, nuclear pyknosis and apoptotic bodies formation. The results of cytometry showed the typical apoptotic peak.</p><p><b>CONCLUSION</b>TNF-alpha and cycloheximide together could inhibit MC proliferation and induce MC apoptosis.</p>
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Técnicas In Vitro
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Fator de Necrose Tumoral alfa
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Apoptose
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Cicloeximida
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Biologia Celular
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Proliferação de Células
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Sinergismo Farmacológico
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Melanócitos
Limite:
Adolescent
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Adult
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2005
Tipo de documento:
Article