Ultrastructural analysis of glioma stem cells-progenitors / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 663-667, 2008.
Article
em Zh
| WPRIM
| ID: wpr-255607
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>It is well known that glioma stem cells-progenitors (GSCP) proliferate indefinitely and hardly differentiate in vitro, however, the reasons remain unknown. The aim of this study was to explore the ultrastructural basis of GSCP.</p><p><b>METHODS</b>GSCP, kept by our laboratory, were collected, embedded, and cut into ultrathin sections and observed under the transmission electron microscope.</p><p><b>RESULTS</b>A single GSCP usually had relatively well developed mitochondria, Golgi apparatuses, ribosomes, and undeveloped rough endoplasmic reticulum, but seldom lysosomes and no typical autophagosomes were found, and the nuclear-cytoplasmic ratio was high. The nuclei frequently contained huge amounts of euchromatin and a small quantity of heterochromatin, and in most nuclei there were only one nucleolus, however, two or more nucleoli were also common. Typical apoptotic cells could hardly be found in tumor-spheres, and between neighboring cells in tumor-spheres there were incompletely developed desmosomes or intermediate junction.</p><p><b>CONCLUSION</b>The ultrastructural features of glioma stem cells-progenitors showed that BTSCP were very primitive and the lack of autophagy and the underdevelopment of some other cellular organelles are probably the reasons for the differential inhibition of GSCPs.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Células-Tronco Neoplásicas
/
Neoplasias Encefálicas
/
Cromatina
/
Membrana Celular
/
Núcleo Celular
/
Citoplasma
/
Linhagem Celular Tumoral
/
Microscopia Eletrônica de Transmissão
/
Glioma
/
Junções Intercelulares
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2008
Tipo de documento:
Article