Enhancement of Virus Replication in An Influenza A Virus NS1-Expresssing 293 Cell Line / 生物医学与环境科学（英文）
Biomedical and Environmental Sciences
; (12): 224-228, 2016.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-258830
Biblioteca responsável:
WPRO
ABSTRACT
The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus da Influenza A
/
Fisiologia
/
Cultura de Vírus
/
Replicação Viral
/
Linhagem Celular
/
Regulação da Expressão Gênica
/
Proteínas não Estruturais Virais
/
Células HEK293
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2016
Tipo de documento:
Artigo
