Experimental research of the inhibition of vascular endothelial growth factor C expression in gastric cancer by targeting RNA interference / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 64-67, 2010.
Article
em Zh
| WPRIM
| ID: wpr-259338
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the plasmid expression vector pSIH1-H1-copGFP for RNA interference against vascular endothelial growth factor C (VEGF-C) and to evaluate its effect on the expression of VEGF-C mRNA in gastric cancer cells after transfection.</p><p><b>METHODS</b>Three siRNAs of genome sequence of VEGF-C gene were retrieved from GenBank and one negative chain was used as control. Four siRNAs were cloned into plasmid pSIH1-H1-copGFP,which were then transfected into gastric cancer cells (SGC7901). The expression of VEGF-C mRNA was analyzed by RT-PCR.</p><p><b>RESULTS</b>The recombinant plasmid of pSIH1-H1-copGFP specific for VEGF-C was confirmed by gene sequencing analysis. The target sequence obtained was completely consistent with the design. Transfection efficiency of the three siRNAs ranged from 60% to 70%. After transfection, the expression of VEGF-C mRNA in SGC7901 cells was significantly inhibited. Inhibition rates of VEGF-C mRNA expression were 35.4%, 33.8% and 81.5% in the three siRNA plasmid vectors, respectively.</p><p><b>CONCLUSION</b>The siRNA expression plasmid vector against VEGF-C mRNA is successfully constructed, and RNAi may be a useful technique to inhibit the lymphangiogenesis of gastric cancer.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Plasmídeos
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Neoplasias Gástricas
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Transfecção
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RNA Interferente Pequeno
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Interferência de RNA
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Linhagem Celular Tumoral
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Fator C de Crescimento do Endotélio Vascular
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Vetores Genéticos
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2010
Tipo de documento:
Article