Induction of UGT1A1 expression by praeruptorin A and praeruptorin C through hCAR pathway / 药学学报
Acta Pharmaceutica Sinica
; (12): 794-798, 2013.
Article
em Zh
| WPRIM
| ID: wpr-259548
Biblioteca responsável:
WPRO
ABSTRACT
This study is purposed to investigate the effects of praeruptorin A (PA) and praeruptorin C (PC) on UGT1A1 in HepG2 cells through hCAR pathway. PA and PC were incubated with HepG2 cells for 24 h and 48 h, mRNA and protein expressions of UGT1A1 were determined by real-time PCR and Western blotting assays. Additionally, effects of PA and PC on UGT1A1 mRNA and protein expressions were also measured after transient transfection of a specific CAR siRNA for 72 h in HepG2 cells. UGT1A1 mRNA and protein expression levels were significantly increased by PA and PC after incubation for 48 h. Moreover, the mRNA and protein up-regulations of UGT1A1 were attenuated by transient transfection of a specific CAR siRNA, suggesting the induction was mediated by CAR. The results suggest that PA and PC can significantly up-regulate UGT1A1 expression partially via the CAR-mediated pathway.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Plantas Medicinais
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RNA Mensageiro
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Medicamentos de Ervas Chinesas
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Transfecção
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Transdução de Sinais
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Química
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Glucuronosiltransferase
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Receptores Citoplasmáticos e Nucleares
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Raízes de Plantas
Limite:
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2013
Tipo de documento:
Article