Impact and potential mechanism of human α-defensin 1 on low-density lipoprotein oxidation ability of ECV304 cells / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 406-410, 2013.
Article
em Zh
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| ID: wpr-261542
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role and potential mechanism of human α-defensin 1 (HNP-1) on low-density lipoprotein (LDL) oxidation ability of human endothelial cells (EVC304).</p><p><b>METHODS</b>Post incubation with LDL for 3 h, the malondialdehyde (MDA) and protein carbonyl (PCO) were detected in untreated ECV304 (control) and in HNP-1 transfected ECV304 in the presence and absence of siRNA against HNP-1. Flow cytometry and fluorescence microscopy were used to detect the generation of oxygen free radical in the ECV304 which have been pretreated by LDL, LPS and HNP-1, respectively.</p><p><b>RESULT</b>Compared with control group, MDA level was significantly increased in HNP-1 transfected [(4.21 ± 0.03) vs. (3.15 ± 0.02) nmol/mg · pro] or in HNP-1 stimulated ECV304 cells [(14.49 ± 1.10) vs. (9.47 ± 1.18) nmol/mg · pro], which could be significantly downregulated by siRNA [(3.76 ± 0.48) vs. (4.54 ± 0.28) nmol/mg·pro, all P < 0.05]. PCO was also significantly increased in HNP-1 transfected ECV304 cells. The levels of free radical were significantly increased in HNP-1 transfected or HNP-1 stimulated ECV304 cells.</p><p><b>CONCLUSION</b>HNP-1 can enhance the LDL oxidation ability of human endothelial cells via promoting the generation of free radicals.</p>
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Base de dados:
WPRIM
Assunto principal:
Transfecção
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Linhagem Celular
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Alfa-Defensinas
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RNA Interferente Pequeno
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Células Endoteliais
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Genética
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Lipoproteínas LDL
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2013
Tipo de documento:
Article