Effect of carbon monoxide and nitric oxide on the apoptosis of hippocampal neurons in rats with febrile seizures / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 231-234, 2006.
Article
em Zh
| WPRIM
| ID: wpr-262737
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Febrile seizure (FS) is the most common type of seizure disorders in children. Recurrent FS can cause hippocampal neurons injury. At the same time heme oxygenase/carbon monoxide (HO/CO) system and nitric oxide synthase/nitric oxide (NOS/NO) system were up-regulated and interacted each other. This study examined the effects of the two systems on the apoptosis of hippocampal neurons in rats with recurrent FS.</p><p><b>METHODS</b>FS was induced in rats by exposure to warm water bath (45.2 degrees C), once every 2 days, 10 times in all. Sprague-Dawley (SD) rats aged 21 days were randomly assigned into four groups Control (37 degrees C water bath exposure), FS, FS + ZnPP-IX (HO inhibitor) and FS + L-NAME (NOS inhibitor) groups. The apoptosis of hippocampal CA1 neurons was detected by TUNEL.</p><p><b>RESULTS</b>After recurrent FS, the apoptotic cells in the hippocampal CA1 neurons increased by 225% compared with those in the Control group (P < 0.01). The apoptotic cells in the FS+ZnPP-IX group increased by 62% and 425% compared with those in the FS and the Control groups (both P < 0.01). The apoptotic cells in the FS + L-NAME group decreased by 38% compared with those in the FS group (P < 0.01) and increased by 100% compared with those in the Control group (P < 0.05).</p><p><b>CONCLUSIONS</b>In recurrent FS, exogenous administration of HO inhibitor ZnPP-IX may induce an increase of apoptotic cells in hippocampal neurons, while NOS inhibitor L-NAME may decrease the apoptotic cells. The results suggest that the HO/CO system might alleviate neuronal damage, while NOS/NO system might augment neuronal damage.</p>
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Fisiologia
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Monóxido de Carbono
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Ratos Sprague-Dawley
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Apoptose
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NG-Nitroarginina Metil Éster
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Convulsões Febris
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Marcação In Situ das Extremidades Cortadas
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Tratamento Farmacológico
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2006
Tipo de documento:
Article