Experimental study on the effects of inactivated and un-inactivated pharmaco-serum of diabetic rats fed with Chinese herbs Qianggubao decoction on the proliferation of osteoblast cultured in vitro / 中国骨伤
China Journal of Orthopaedics and Traumatology
; (12): 429-431, 2008.
Article
em Zh
| WPRIM
| ID: wpr-263724
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of inactivated and un-inactivated pharmaco-serum of diabetic rats fed with Chinese herbs Qianggubao decoction on the proliferation of osteoblast cells (OB)cultured in vitro.</p><p><b>METHODS</b>OB was isolated from the skull of newly born SD rats aged 1 to 2 days by means of Trypsin-collagenase digestion and identified by image analysis under inverted microscope, V-G collagen staining, ALP staining, calcification nod staining etc. After the OB was identified, in activated and un-inactivated pharmaco-serum of diabetic rats fed with Qianggubao decoction of ferent phase (rats were fed with medicine 3 days or 5 days after last fed with medicine 1 hour or 3 hours) and concentration (5%, 10%, 20%) were added to the OB and incubated. After determined times, the effects of the proliferation of osteoblasts were detected by MTT analysis.</p><p><b>RESULTS</b>There was significant difference between un-inactivated pharmaco-serum and inactivated pharmaco-serum on the proliferation of osteoblasts, and un-inactivated serum had stronger effects to improve the proliferation of osteoblasts (P < 0.01 or P < 0.05).</p><p><b>CONCLUSION</b>Un-inactivated and inactivation pharmaco-serum of diabetic rats fed with Chinese herbs Qianggubao decoction can influence the proliferation of, and the un-inactivated pharmaco-serum has stronger effects.</p>
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Base de dados:
WPRIM
Assunto principal:
Osteoblastos
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Farmacologia
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Fisiologia
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Sangue
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Medicamentos de Ervas Chinesas
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Células Cultivadas
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Ratos Sprague-Dawley
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Proliferação de Células
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Diabetes Mellitus Experimental
Limite:
Animals
Idioma:
Zh
Revista:
China Journal of Orthopaedics and Traumatology
Ano de publicação:
2008
Tipo de documento:
Article