Enrichment of saponins in Radix et Rhizoma Cynanchi Atrati with macroporous resin / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1390-1393, 2008.
Article
em Zh
| WPRIM
| ID: wpr-264874
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop an approach to the determination of saponins in Radix Cynanchi Atrati, and to optimize the parameters for purified the preparation of total saponins by macroporous resin column chromatography.</p><p><b>METHOD</b>Using cynanversicoside A as a reference, the determination of saponins was performed; according to the elution rate and the purity of the products, the preparation performance of total saponins by macroporous resin was investigated, and its parameters were optimized.</p><p><b>RESULT</b>The saponins in Radix Cynanchi Atrati were successfully determined at 518 nm by vanillin-perchloric acid as spray reagent. The macroporous resin HP-20 showed static absorption ratio of 59. 3 mg x g(-1); the 70% ethanol extraction of Radix Cynanchi Atrati was eluted from column of macroporous resin HP-20 by water and 30% ethanol, and the saponins were concentrated in 90% ethanol solution. The content of saponin part eluted from HP-20 column was 77.62%.</p><p><b>CONCLUSION</b>The proposed approach allows convenient and efficient preparation and purification of saponin in Radix Cynanchi Atrati.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Resinas Vegetais
/
Saponinas
/
Benzaldeídos
/
Calibragem
/
Química
/
Reprodutibilidade dos Testes
/
Porosidade
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Sensibilidade e Especificidade
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Percloratos
/
Cynanchum
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2008
Tipo de documento:
Article