Isolation and identification of reprogramming genes related to the development of the rabbit reconstructed embryos / 生物工程学报
Chinese Journal of Biotechnology
; (12): 30-34, 2003.
Article
em Zh
| WPRIM
| ID: wpr-270043
Biblioteca responsável:
WPRO
ABSTRACT
By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT-PCR), 25 reprogramming cDNA fragments were obtained from single 2-cell, 8-cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse-Northern and characterized with stage-specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fisiologia
/
Blastocisto
/
Northern Blotting
/
Reação em Cadeia da Polimerase
/
Regulação da Expressão Gênica no Desenvolvimento
/
Desenvolvimento Embrionário
/
Embrião de Mamíferos
/
Genética
/
Metabolismo
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Pregnancy
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2003
Tipo de documento:
Article