Effect of NECL1 on the proliferation of T98G glioma cell line / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 280-283, 2008.
Article
em Zh
| WPRIM
| ID: wpr-270706
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the regulation role of tumor suppressor NECL1 on the proliferation of glioma cell line.</p><p><b>METHODS</b>We detected the expression level of NECL1 in human normal brain tissue and glioma cell lines using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot. T98G cell line in which NECL1 was silent and whose transfection efficiency was relatively high as target cell was chosen, and the effect of NECL1 on the proliferation of T98G cell line in vitro was detected by using cell growth curve, flow cytometry, and Hoechst staining.</p><p><b>RESULTS</b>NECL1 was abrogated or markedly reduced in 6 glioma cell lines. When NECL1 was overexpressed in T98G cell line, the cell growth rate obviously decreased and the number of apoptotic cells remarkably increased when compared with the control group.</p><p><b>CONCLUSION</b>NECL1 may inhibit the proliferation of T98G cells by inducing its apoptosis.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Fisiologia
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Técnicas In Vitro
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Imunoglobulinas
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Transfecção
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Western Blotting
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Apoptose
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Linhagem Celular Tumoral
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Proliferação de Células
Limite:
Humans
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2008
Tipo de documento:
Article