Analysis of c-kit gene mutations in gastrointestinal stromal tumors / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 89-92, 2004.
Article
em Zh
| WPRIM
| ID: wpr-271059
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To define the frequency and spectrum of c-kit gene mutations in gastrointestinal stromal tumors (GIST).</p><p><b>METHODS</b>Fifty two cases of GIST and 28 cases of other tumors were examined for mutations in exon 11, 9 and 13 of c-kit gene using PCR amplification and DNA sequencing.</p><p><b>RESULTS</b>Fourteen out of 25 malignant GIST (56%), while 2 of 27 benign and borderline GIST (7.4%) revealed mutations in exon 11 of c-kit gene (P < 0.01). Most of the mutations consisted of in-frame deletion or replication from 3 to 48 bp in heterozygous and homozygous fashions, but none of the mutations disrupted the downstream reading frame of the gene. Point mutation and deletion concentrated at 550 - 570 codons but replication clustered within 570 - 585 codons. The mutation pattern in recurrence tissues was the same as the primary ones. Normal tissues adjacent to GIST with or without c-kit gene mutations showed wild type c-kit gene sequence. No mutation was found in exon 9 and 13. Neither c-kit gene expression nor gene mutations was found in 3 leiomyomas, 8 leiomyosarcomas, 2 schwannomas, 2 intra-abdomenal fibromitoses and 8 adenocarcinomas.</p><p><b>CONCLUSION</b>The mutations in exon 11 of c-kit gene might partially represent one of the molecular mechanisms of GIST. It can be used as a marker for distinguishing benignancy and malignancy of GIST. The mutations did not involve the reading frame. Except for long frame deletion, most mutations also did not affect protein expression. Mutation of c-kit gene in GIST provides a new genotypic marker to distinguish GIST from authentic leiomyomas, leiomyosarcomas, schwannomas and etc.</p>
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1
Base de dados:
WPRIM
Assunto principal:
Proto-Oncogenes
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Dados de Sequência Molecular
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Sequência de Bases
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Proteínas Proto-Oncogênicas c-kit
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Neoplasias Gastrointestinais
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Genética
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Mutação
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2004
Tipo de documento:
Article