Effect of PDK1 on Neutrophil Differentiation / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 932-935, 2017.
Article
em Zh
| WPRIM
| ID: wpr-271892
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio, number and differentiation of neutrophil.</p><p><b>METHODS</b>The PDK1 expression level of Vav-Cre;PDK1mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis.</p><p><b>RESULTS</b>The RNA expression level of bone marrow cells of Vav-Cre;PDK1mouse was dramatically lower than that of the control mouse. The number of functional GMP was lower in Vav-Cre;PDK1mouse in contrast to controls. The percentage and number of neutrophil were lower, but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre;PDK1group compared with control groups.</p><p><b>CONCLUSION</b>PDK1 affects the process of the differention of hematopoietic stem cells to GMP, the neutrophil differention and maturation.</p>
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2017
Tipo de documento:
Article