Quality evaluation of prepared slices of Paeonia lactiflon--determination of paeoniflorin by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the quality criteria of the prepared slices of Paeonia lactiflon.</p><p><b>METHOD</b>RP-HPLC was used for the determination of paeoniflorin in 10 lots of samples by ultrasound-assisted extraction.</p><p><b>RESULT</b>The samples were extracted with 50% methanol. Seperation of the solution was performed on an ODS column with a mobile phase of acetonitrile-water (1882), detected at 230 nm.</p><p><b>CONCLUSION</b>The method is simple, repeatable, accurate and applicable.</p>