Effect of bushen tongmai recipe on insulin resistance and ovulation dysfunction in PCOS rats accompanied with insulin resistance / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 733-736, 2009.
Article
em Zh
| WPRIM
| ID: wpr-273632
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rat model of polycystic ovarian syndrome accompanied with insulin resistance (PCOS-IR), and to observe the effects of Bushen Tongmai Recipe (BSTMR) on insulin resistance and ovulation dysfunction in the model.</p><p><b>METHODS</b>Sodium prasterone sulfate at the daily dose of 9 mg/100 g was subcutaneously injected to female SD rats, 23-day old, for 20 days, and fed with high-fat forage for 80 days to establish the rat model of PCOS-IR. The model rats were randomized into the model group and the treated group administered with BSTMR. Besides, a group consisted of 15 healthy rats was set up as a normal control. Ovarian histological changes and ovulation condition in rats were examined with hematoxylin and eosin (HE) staining; fasting blood glucose (FBG) was determined by glucose oxidase method; serum levels of fasting insulin (FINS), serum testosterone (T), estradiol (E2), progesterone (P), follicle stimulating hormone (FSH) and luteotrophic hormone (LH) were detected by radioimmunoassay (RIA).</p><p><b>RESULTS</b>Compared with the normal group, in the model group, the mean number of corpus luteum, ovulation rate, FSH level and insulin sensitive index (ISI) were lower significantly (P <0.01), and levels of FBG, FINS, T, E2 and LH were higher (P<0.05, P <0.01). Compared with the model group, in the treated group, the mean number of corpus luteum, ovulation rate, levels of FSH and ISI were higher, and levels of FINS, T and E, were lower (all P <0.01).</p><p><b>CONCLUSION</b>Rat model of PCOS-IR could be established by sodium prasterone sulfate and high-fat forage, and the insulin resistance and ovulation dysfunction in the model rats could be improved by BSTMR.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Ovulação
/
Farmacologia
/
Síndrome do Ovário Policístico
/
Sangue
/
Resistência à Insulina
/
Medicamentos de Ervas Chinesas
/
Modelos Animais de Doenças
/
Insulina
/
Metabolismo
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2009
Tipo de documento:
Article