Antibacterial activity and mechanism of baicalein / 药学学报
Acta Pharmaceutica Sinica
; (12): 1587-1592, 2012.
Article
em Zh
| WPRIM
| ID: wpr-274618
Biblioteca responsável:
WPRO
ABSTRACT
Baicalein (BAI) is an effective bactericide. The antibacterial activity and mechanism experiments were carried out by determining conductivity and content of macromolecules of membrane penetrability, the oxidative respiratory metabolism and protein synthesis changes and the inhibition of DNA topoisomerase activities. Electrical conductivity and the number of large molecules of BAI increased 2.48% and 1.8%, respectively, than that of the control. However, the membrane integrity did not destroyed by BAI directly. With BAI treatment, inhibition rates of activities for SDH and MDH were 56.2% and 57.4%, respectively, demonstrating that BAI could inhibit cell respiratory. After treated with BAI for 20 h, the total soluble content of proteins decreased by 42.83%. Moreover, the activities of DNA topoisomerase I and II were inhibited completely by 0.2 mmol x L(-1) BAI. These results indicated that BAI had obvious antibacterial activity on Staphylococcus aureus. The mechanism is that it could affect bacterial membrane penetrability, inhibit protein synthesis and influence SDH, MDH and DNA topoisomerase I and II activities to exert its antibacterial functions.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Plantas Medicinais
/
Solubilidade
/
Staphylococcus aureus
/
Succinato Desidrogenase
/
Proteínas de Bactérias
/
Permeabilidade da Membrana Celular
/
Química
/
DNA Topoisomerases Tipo II
/
DNA Topoisomerases Tipo I
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2012
Tipo de documento:
Article