Tea polyphenols reduces the apoptosis of spermatogenic cells in rats with experimental varicocele / 中华男科学杂志
National Journal of Andrology
; (12): 702-707, 2015.
Article
em Zh
| WPRIM
| ID: wpr-276034
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of tea polyphenols (TP) on the apoptosis of germ cells in rats with experimental varicocele.</p><p><b>METHODS</b>Thirty-two adolescent male Wistar rats were randomly and equally divided into groups A (sham-operation), B (high-dose TP), C (low-dose TP), and D (experimental left varicocele). Experimental varicocele was induced by partial ligation of the left renal vein in the latter three groups of rats. The animals in groups A and D were fed with normal saline, while those in B and C with TP at 40 and 10 mg per kg per d, respectively, all for 4 weeks. Then, all the rats were sacrificed and the left testes harvested for determination of the expression of HIF-1, Bcl-2, Bax, CytC, and caspase-3 by immunohistochemistry and measurement of the apoptosis index (AI) of spermatogenic cells.</p><p><b>RESULTS</b>The expression of Bcl-2 was higher in groups B and C than in D but lower than in A (P < 0.05), and lower in C than in B (P < 0.05). However, the expressions of HIF-1, Bax, CytC, and caspase-3 were lower in groups B and C than in D but higher than in A (P < 0.05), and higher in C than in B (P < 0.05). The AI of spermatogenic cells was the lowest in group A, higher in D than in the other groups but lower in B than in C (P < 0.05).</p><p><b>CONCLUSION</b>TP can reduce the apoptosis of spermatogenic cells in a dose-dependent manner in varicocele rats.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Veias Renais
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Espermatozoides
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Chá
/
Testículo
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Varicocele
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Distribuição Aleatória
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Química
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Ratos Wistar
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Apoptose
Limite:
Animals
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2015
Tipo de documento:
Article