Molecular identification of heparin from pigs by allele-specific PCR (AS-PCR) and amplification refractory mutation system (ARMS) / 药学学报
Acta Pharmaceutica Sinica
; (12): 535-541, 2008.
Article
em Zh
| WPRIM
| ID: wpr-277817
Biblioteca responsável:
WPRO
ABSTRACT
The aim of this study is to develop a convenient and effective method for the identification of heparin from pigs (include Sus scrofa domestica Brisson and Sus scrofa riukiuanus). Based on sequences of D-loop region of pigs and the other animals, two pairs of highly specific primers were designed for distinguishing heparin of pigs from other animals. The primers were employed to amplify D-loop region of DNA templates extracted from pig and seven other animal species that amounted to 49 samples. AS-PCR (allele-specific PCR) and ARMS (amplification refractory mutation system) were all suitable for fast identification of heparin from pig with anneal temperature at 54-56 degrees C in AS-PCR and with wider anneal temperature in ARMS,at 52-58 degrees C. An about 170 bp DNA fragments were amplified from separately pigs and whereas no DNA fragment was amplified from other samples under the same reaction condition.
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Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Controle de Qualidade
/
Ruminantes
/
DNA Mitocondrial
/
Análise Mutacional de DNA
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Heparina
/
Reação em Cadeia da Polimerase
/
Contaminação de Medicamentos
Limite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2008
Tipo de documento:
Article