Effect of hepatitis B virus X protein on suppressor of cytokine signaling-1 expression / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 723-727, 2013.
Article
em Zh
| WPRIM
| ID: wpr-277999
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hepatitis B virus-encoded X protein (HBx) on the expression of host-encoded suppressor of cytokine signaling-1 (SOCS-1) and to explore the possibility of an underlying mechanism involving modulation of CpG island methylation in the SOCS-1 gene promoter.</p><p><b>METHODS</b>The immortalized human derived non-tumor liver cell line QSG7701 was transfected with a recombinant HBx plasmid (pcDNA-X) or an empty vector control plasmid (pcDNA3.0) and stably transfected clones were selected by G418 resistance screening. Untransfected cells served as negative controls. Expression of SOCS-1 mRNA and protein was detected by real-time quantitative PCR and western blotting. The methylation status of SOCS-1 was detected by methylation-specific PCR (MSP). The significance of intergroup differences was analyzed by one-way ANOVA or pairwise comparison with post-hoc LSD test.</p><p><b>RESULTS</b>SOCS-1 mRNA level was significantly lower in the pcDNA-X/QSG7701 cells compared to that in the pcDNA3.0/QSG7701 and untransfected cells (0.3249+/-0.0536 vs. 1.0543+/-0.1937 and 1.00; F = 19.6, P = 0.042). SOCS-1 protein level was similarly lower in the pcDNA-X/QSG7701 cells (0.1496+/-0.0106 vs. 0.1984+/-0.0438 and 0.2152+/-0.0816; F = 19.4, P = 0.048). The SOCS-1 promoter region showed methylation only in the pcDNA-X/QSG7701 cells.</p><p><b>CONCLUSION</b>HBx-expressing human hepatocytes have down-regulated SOCS-1 expression, both at the mRNA and protein levels, and this effect corresponds to increased methylation in the SOCS-1 promoter region harboring CpG islands.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plasmídeos
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RNA Mensageiro
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Transfecção
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Transativadores
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Linhagem Celular
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Regiões Promotoras Genéticas
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Ilhas de CpG
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Metilação de DNA
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Proteínas Supressoras da Sinalização de Citocina
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Proteína 1 Supressora da Sinalização de Citocina
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2013
Tipo de documento:
Article