Permeability of blood-brain barrier oxygen-glucose deprivation induced by tetramethylpyrazine-puerarin in vitro / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2745-2748, 2010.
Article
em Zh
| WPRIM
| ID: wpr-279361
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore permeability of artificial blood-brain barrier (aBBB) by oxygen-glucose deprivation combined (OGD)-induced using tetramethylpyrazine combined with puerarin in vitro.</p><p><b>METHOD</b>Rats were divided into normal control group, model group, tetramethylpyrazine group, puerarin group, tetramethylpyrazine-puerarin group and nimodipine group. Culture rat brain microvascular endothelial cells and astrocytes in vitro and build the OGD-induced aBBB damage model. Evaluate aBBB damage characteristics by TEER, gamma-GT, AKP and LDH. Determine contents of tetramethylpyrazine, puerarin, nimodipine and calculate drug permeating concentration of OGD-induced aBBB model by HPLC.</p><p><b>RESULT</b>Compared with the model, the level of TEER was lower than the control group with significant difference (P < 0.01). The levels of gamma-GT, AKP in tetramethylpyrazine group, tetramethylpyrazine-puerarin group and nimodipine group were higher than the model group, the differences were significant (P < 0.01). Compared with tetramethylpyrazine group or puerarin group, the level of AKP of tetramethylpyrazine-puerarin group increased significantly (P < 0.01). The differences of levels of TEER, gamma-GT, AKP and LDH between tetramethylpyrazine-puerarin group and nimodipinthe group were significant (P < 0.05). Tetramethylpyrazine-puerarin group has a synergistic effect of increasing TEER, gamma-GT, AKP and reducing LDH. The permeating rate in tetramethylpyrazine-puerarin group was higher than tetramethylpyrazine group and puerarin group.</p><p><b>CONCLUSION</b>Tetramethylpyrazine-puerarin can permeate aBBB more easily and protect aBBB. The cause may relate to reducing the permeability of the OGD-induced aBBB.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Oxigênio
/
Permeabilidade
/
Farmacologia
/
Fisiologia
/
Pirazinas
/
Barreira Hematoencefálica
/
Ratos Sprague-Dawley
/
Combinação de Medicamentos
/
Glucose
/
Isoflavonas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Article