Detection of an NA gene molecular marker in H7N9 subtype avian influenza viruses by pyrosequencing / 病毒学报
Chinese Journal of Virology
; (6): 369-374, 2014.
Article
em Zh
| WPRIM
| ID: wpr-280358
Biblioteca responsável:
WPRO
ABSTRACT
This study aimed to establish a method for the detection and identification of H7N9 avian influenza viruses based on the NA gene by pyrosequencing. According to the published NA gene sequences of the avian influenza A (H7N9) virus, a 15-nt deletion was found in the NA gene of H7N9 avian influenza viruses. The 15-nt deletion of the NA gene was targeted as the molecular marker for the rapid detection and identification of H7N9 avian influenza viruses by pyrosequencing. Three H7N9 avian influenza virus isolates underwent pyrosequencing using the same assay, and were proven to have the same 15-nt deletion. Pyrosequencing technology based on the NA gene molecular marker can be used to identify H7N9 avian influenza viruses.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Doenças das Aves Domésticas
/
Proteínas Virais
/
Virologia
/
Aves
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Galinhas
/
Classificação
/
Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2014
Tipo de documento:
Article