Genetic polymorphisms in the promoter region of cyclooxygenase-2 and their association with risk of gastric cancer / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 119-123, 2006.
Article
em Zh
| WPRIM
| ID: wpr-281248
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the genetic polymorphisms in the promoter region of cyclooxygenase-2 ( COX-2) and evaluate their association with the risk of gastric cancer.</p><p><b>METHODS</b>Single-strand conformational polymorphism and DNA sequencing were used to screen the genetic variants of the COX-2 promoter region. Total 323 patients with gastric cancer and 646 frequency-matched controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Odds ratios (OR) and 95% confidence intervals (CI) were estimated by logistic regression. Haplotype frequency was estimated using Haplo. stat software.</p><p><b>RESULTS</b>Three single nucleotide polymorphisms, including - 1290A > G, -1195G > A, and -765G > C were identified in the promoter of COX- 2. Case-control analysis showed an increased risk of developing gastric cancer for the -1290AG (OR 1.64; 95% CI 1.03-2.61), -1195AA (OR 2.68; 95% CI 1.65-4.33), and -765CG (OR 2.66; 95% CI 1.53-4.64) genotype carriers, respectively, compared with noncarriers. A greater risk of developing gastric cancer was observed for the A(-1290) -A(-1195) -C(-765) haplotype compared with the A(-1290) -G(-1195) -G(-765) haplotypes (OR 11.80; 95% CI 2.43-57.25).</p><p><b>CONCLUSION</b>Genetic polymorphisms in COX-2 promoter region may play a role in gastric carcinogenesis.</p>
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Base de dados:
WPRIM
Assunto principal:
Polimorfismo Genético
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Neoplasias Gástricas
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Haplótipos
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Reação em Cadeia da Polimerase
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Risco
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Regiões Promotoras Genéticas
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Predisposição Genética para Doença
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Polimorfismo de Nucleotídeo Único
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Ciclo-Oxigenase 2
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Genética
Tipo de estudo:
Etiology_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2006
Tipo de documento:
Article