Capacity of HIV enzyme immunoassay diagnostic kits to detect antibodies against different genotypes of HIV / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the capacity of commercial HIV enzyme immunoassay (EIA) diagnostic kits to detect antibodies against different genotypes of HIV.</p><p><b>METHODS</b>HIV RNA was detected with RT-PCR from samples positive for HIV antibody. The purified PCR products were sequenced directly and the genotypes of HIV from samples were analyzed. The samples for each genotype of HIV were diluted and the diluted samples were detected with different HIV EIA diagnostic kits.</p><p><b>RESULTS</b>All 20 samples positive for HIV antibody were also positive for HIV RNA; 9 of 20 isolates were genotype B, 9 of them were genotype C or CRF BC, 2 of them were CRF AE. The sensitivity of different HIV EIA diagnostic kits to detect antibodies against different genotypes of HIV was not significantly different.</p><p><b>CONCLUSION</b>The capacity of commercial HIV diagnostic kits to detect antibodies against different HIV genotypes may not be significantly different.</p>