Cloning, expression and immunocharacterization of the capsid protein of human Norwalk virus Guangzhou strain NVgz01 / 南方医科大学学报
Journal of Southern Medical University
; (12): 1410-1413, 2007.
Article
em Zh
| WPRIM
| ID: wpr-283118
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clone, express and characterize the capsid protein of human Norwalk virus Guangzhou strain NVgz01.</p><p><b>METHODS</b>On the basis of successful construction of full-genome clones and sequence analysis of human norovirus Guangzhou strain NVgz01, the full capsid gene was ligated into pET28a (+) for expression. After IPTG induction, the recombinant protein was purified through metal (Ni(2+)) chelating affinity chromatography. Western blotting and enzyme-linked immunosorbent assay (ELISA) were used to determine the antigenicity of the recombinant protein.</p><p><b>RESULTS</b>The recombinant capsid gene was overexpressed in E.coli, yielding the recombinant protein with relative molecular mass of 62x10(3) that was highly purified through metal (Ni(2+)) chelating affinity chromatography. IDEIA Norovirus Kit and immunoassay showed that the recombinant protein had good antigenicity.</p><p><b>CONCLUSION</b>The capsid gene of norovirus Guangzhou strain has been cloned and expressed, which can be useful for developing diagnostic reagents or vaccine of norovirus.</p>
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Base de dados:
WPRIM
Assunto principal:
Plasmídeos
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Ensaio de Imunoadsorção Enzimática
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Expressão Gênica
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Western Blotting
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Clonagem Molecular
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Vírus Norwalk
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Proteínas do Capsídeo
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Alergia e Imunologia
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Article