Protective effect of epidermal growth factor on isograft mucosal structure after small bowel transplantation in rat / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 448-451, 2006.
Article
em Zh
| WPRIM
| ID: wpr-283298
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of epidermal growth factor(EGF) on mucosal structure after small bowel transplantation in rat.</p><p><b>METHODS</b>Total small bowel transplantation was performed in inbred Wistar(RT1k) rats heterotopically, either total parenteral nutrition(control group, n=10) or TPN supplemented with epidermal growth factor(EGF group, n=10) was given to the recipients from the second day to 10th day after operation. The changes of morphology, ultrastructure, mucosal protein and DNA contents were determined.</p><p><b>RESULTS</b>The height of mucosal villus,the depth of crypt, the thickness of mucosa and the surface area of villus were (284.47+/-31.58)microm, (98.78+/-10.83 microm, (389.56+/-31.72)microm and (0.089+/-0.009 )mm(2) respectively in EGF group,and (176.45+/-14.62)microm, (74.45+/-8.34)microm, (259.38+/-24.65) microm, and (0.041+/-0.005)mm2 respectively in the control group. The morphological parameters were significantly higher in EGF group than those in the control group. Mucosal protein content was (84.65+/-8.32)mg/g wet wt in EGF group,significantly higher than (53.73+/- 11.45) mg/g wet wt in the control group(P=0.012). DNA content was also significantly higher in EGF group than that in the control group. [(0.86+/0.10)mg/g wet wt vs (0.51+/-0.06)mg/g wet wt, P=0.008]. Nearly normal ultrastructure of the graft enterocyte was maintained in EGF group, while atrophic microvilli and broken mitochondrial crista were observed in the control group.</p><p><b>CONCLUSION</b>EGF can preserve the mucosal structure of the graft, maintain the integrity of the ultrastructure of graft enterocyte after small bowel transplantation in rat.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Transplante
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Transplante Autólogo
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Nutrição Parenteral Total
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Ratos Wistar
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Transplantes
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Usos Terapêuticos
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Fator de Crescimento Epidérmico
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Mucosa Intestinal
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Intestino Delgado
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2006
Tipo de documento:
Article