Study of mutations in 5'-noncoding region of BCL-6 gene in B-cell non-Hodgkin's lymphomas / 中华病理学杂志
Chinese Journal of Pathology
; (12): 238-241, 2004.
Article
em Zh
| WPRIM
| ID: wpr-283539
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate BCL-6 gene mutations in B-cell non-Hodgkin lymphomas (B-NHL) and their implications in lymphoma pathogenesis.</p><p><b>METHODS</b>Polymerase chain reaction (PCR) and direct DNA sequencing methods were used to identify mutations in the 5'-noncoding region of BCL-6 gene in 135 cases of B-NHL, 5 cases of T-NHL, 5 cases of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and 10 cases of reactive hyperplasia of lymph node.</p><p><b>RESULTS</b>Mutations were identified in 6 cases of nodal DLBCL (27.3%), 4 cases of FL (22.2%), 4 cases of MALT lymphoma (22.2%), 4 cases of extranodal DLBCL (20.7%) and 2 cases of LRH (20%). No mutations were detected in T-NHL and NLPHL (P < 0.05). There were no significant differences in incidences of BCL-6 gene mutations between nodal and extranodal DLBCL (P > 0.05). All mutations were base substitutions and the frequency of single-base change was 0.14 x 10(-2)/bp approximately 0.68 x 10(-2)/bp.</p><p><b>CONCLUSIONS</b>Mutations of the 5'non-coding region of BCL-6 gene may be involved in the pathogenesis and progression of B-NHL. Molecular demonstration of such mutations may provide a marker of lymphomas derived from the germinal center-related B cells.</p>
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1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Fatores de Transcrição
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Linfoma não Hodgkin
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Dados de Sequência Molecular
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Sequência de Bases
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Linfoma de Células B
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Proteínas Proto-Oncogênicas
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Mutação Puntual
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Regiões 5' não Traduzidas
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Proteínas de Ligação a DNA
Tipo de estudo:
Prognostic_studies
Limite:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2004
Tipo de documento:
Article