Effect of osthole on bone metabolism in rat femoral tissues in vitro / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 561-566, 2013.
Article
em Zh
| WPRIM
| ID: wpr-285960
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of osthole on bone metabolism in rat femoral tissues in vitro.</p><p><b>METHODS</b>The rat femoral tissues were isolated in vitro. The optimal concentrations of ostehole (1×10(-5) mol/L) and estradiol (1×10(-8) mol/L) (the positive control) were selected by alkaline phosphatase activity (ALP). The ALP and calcium levels were detected by commmerical regents, and the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene 2, and bone morphogenetic protein-2 mRNA were determined by real-time reverse transcription-polymerase chain reaction.</p><p><b>RESULT</b>The osthole (1×10(-5) mol/L) significantly increased the activity of ALP, calcium level as well as the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene-2 and bone morphogenetic protein-2 mRNA in rat femoral tissues in vitro.</p><p><b>CONCLUSION</b>Osthole can improve calcium level and ALP activity and regulate the bone metabolism-related genes in rat femoral tissues.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Técnicas In Vitro
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Cálcio
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Ratos Sprague-Dawley
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Cumarínicos
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Fosfatase Alcalina
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Subunidade alfa 1 de Fator de Ligação ao Core
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Ligante RANK
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Osteoprotegerina
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Proteína Morfogenética Óssea 2
Limite:
Animals
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2013
Tipo de documento:
Article