Role of MAPK signal transduction pathway phosphorylation induced by granulocyte-macrophage-colony stimulating factor in the proliferation of hormone-independent prostate cancer / 中华男科学杂志
National Journal of Andrology
; (12): 872-875, 2006.
Article
em Zh
| WPRIM
| ID: wpr-289121
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the phosphorylation intensity of MAPK pathway molecular Erk1/2 and the proliferation of prostate cancer cell line PC-3M.</p><p><b>METHODS</b>Flow cytometry and RT-PCR were employed to study the ratio of different cell cycles and phases, respectively, before and after GM-CSF stimulation. Erk1/2 phosphorylation intensity was examined by Western blot simultaneously.</p><p><b>RESULTS</b>The rate of PC-3M cells at S and G2/M stages and the expression intensity of Ki-67 increased after GM-CSF incubation in a dose-dependent manner. The phosphorylation intensity of Erk1/2 increased remarkably after stimulation with GM-CSF.</p><p><b>CONCLUSION</b>The intensification of Erk1/2 phosphorylation is one important molecular mechanism of the proliferation of hormone-independent prostate cancer.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
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Fosforilação
/
Fisiologia
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Neoplasias da Próstata
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Transdução de Sinais
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Fator Estimulador de Colônias de Granulócitos e Macrófagos
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Antígeno Ki-67
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Proteína Quinase 1 Ativada por Mitógeno
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Quinases de Proteína Quinase Ativadas por Mitógeno
Limite:
Humans
/
Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2006
Tipo de documento:
Article