MicroRNA profiling in patients with hepatocellular carcinoma / 南方医科大学学报
Journal of Southern Medical University
; (12): 976-977, 2010.
Article
em Zh
| WPRIM
| ID: wpr-290015
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differential expression of microRNAs between human hepatocellular carcinoma (HCC) and liver cirrhosis (LC).</p><p><b>METHODS</b>The total RNA was extracted from 25 pairs of HCC and LC tissues. microRNA microarray was used to analyze the microRNA expression profiles, and validation of the microarray results was carried out by real-time quantitative RT-PCR (qRT-PCR).</p><p><b>RESULTS</b>Three microRNAs exhibited higher expression in the HCC samples than in the LC samples. In comparison with the LC samples, the HCC samples showed down-regulated expressions of 9 microRNAs. qRT-PCR verified that has-miR-122a, has-miR-199a, and has-miR-199b were downregulated in HCC, which was in agreement with the microarray results.</p><p><b>CONCLUSION</b>HCC and LC samples have significantly different microRNA expression profiles. These differentially expressed microRNAs may be involved in the pathogensis of HCC.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Regulação Neoplásica da Expressão Gênica
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Carcinoma Hepatocelular
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Perfilação da Expressão Gênica
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MicroRNAs
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Genética
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Cirrose Hepática
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Neoplasias Hepáticas
Limite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Article