Screening of the gene mutation in D-loop region of mitochondrial DNA in oral squamous cell carcinoma / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 285-287, 2013.
Article
em Zh
| WPRIM
| ID: wpr-293619
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the gene mutation in D-loop region of mitochondrial DNA (mtDNA) in oral squamous cell carcinoma (OSCC) tissue and to explore the role of the gene mutation in D-loop region in the OSCC tumorigenesis.</p><p><b>METHODS</b>mtDNA was obtained from cancer, paracancerous and normal mucosa tissues of thirty patients with OSCC. The D-loop regions of mtDNA were amplified with PCR, sequencing and then analyzed by Chromas software and BLAST to identify the mutation site.</p><p><b>RESULTS</b>Mutation in the D-loop region was found in eight cases, with the mutation rate of 27%. There were nine mutations totally, including one point mutation, two base deletions, three insertion mutations, three heterozygous mutations. In these mutations, base deletions were different from each other and heterozygous mutations had no same mutation form, while the three insertion mutations were same, the insertion of base C. One case had T/A heterozygous mutation and base C insertion at the same time.</p><p><b>CONCLUSIONS</b>There were mutations in mtDNA D-loop in OSCC, but the relationship between occurrence of OSCC and mutation of mtDNA needs further study.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
DNA Mitocondrial
/
Neoplasias Bucais
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Carcinoma de Células Escamosas
/
Genética
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Mutação
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Adult
/
Aged
/
Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2013
Tipo de documento:
Article