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Up-regulation of Fas is related to apoptosis of HeLa cells induced by co-immobilized TNF-alpha/IFN-gamma / 生物医学工程学杂志
Article em Zh | WPRIM | ID: wpr-294606
Biblioteca responsável: WPRO
ABSTRACT
This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.
Assuntos
Texto completo: 1 Base de dados: WPRIM Assunto principal: Farmacologia / Células HeLa / Regulação para Cima / Química / Interferon gama / Fator de Necrose Tumoral alfa / Apoptose / Receptor fas / Sinergismo Farmacológico / Proteínas Imobilizadas Limite: Humans Idioma: Zh Revista: Journal of Biomedical Engineering Ano de publicação: 2009 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Farmacologia / Células HeLa / Regulação para Cima / Química / Interferon gama / Fator de Necrose Tumoral alfa / Apoptose / Receptor fas / Sinergismo Farmacológico / Proteínas Imobilizadas Limite: Humans Idioma: Zh Revista: Journal of Biomedical Engineering Ano de publicação: 2009 Tipo de documento: Article