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Ox-LDL down-regulates expression of pigment epithelium-derived factor in human umbilical vein endothelial cells / 生理学报
Acta Physiologica Sinica ; (6): 489-495, 2014.
Article em Zh | WPRIM | ID: wpr-297466
Biblioteca responsável: WPRO
ABSTRACT
Pigment epithelium-derived factor (PEDF) is a multifunctional protein with anti-inflammatory, antioxidant and antithrombotic properties and plays a protective role against atherosclerosis (AS). The purpose of the present study is to explore the effects of oxidized low density lipoprotein (ox-LDL) on the expression of PEDF in cultured human umbilical vein endothelial cells (HUVECs). HUVECs were cultured and incubated with ox-LDL at different concentrations (6.25, 12.5, 25, 50, 100 and 150 mg/L) for 24 h. Apoptosis of endothelial cells were assayed by morphological staining and flow cytometry. The intracellular reactive oxygen species (ROS) levels were measured by flow cytometry. Cell viability was assayed by MTT assay. PEDF protein and mRNA expressions in HUVECs were analyzed by Western blot and quantitative real-time PCR, respectively. The results showed that ox-LDL significantly induced apoptosis, reduced cell viability, increased intracellular ROS levels and decreased the PEDF expression in HUVECs in a concentration-dependent manner. Ox-LDL at 50 mg/L obviously decreased the PEDF protein expression compared with control group (P < 0.05), whereas 25 mg/L ox-LDL already markedly reduced the PEDF mRNA expression (P < 0.05). In conclusion, the results suggest that ox-LDL down-regulates the PEDF expression through an increased ox-LDL-induced intracellular production of ROS.
Assuntos
Texto completo: 1 Base de dados: WPRIM Assunto principal: Farmacologia / Regulação para Baixo / Serpinas / Células Cultivadas / Espécies Reativas de Oxigênio / Apoptose / Biologia Celular / Proteínas do Olho / Células Endoteliais da Veia Umbilical Humana / Lipoproteínas LDL Limite: Humans Idioma: Zh Revista: Acta Physiologica Sinica Ano de publicação: 2014 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Farmacologia / Regulação para Baixo / Serpinas / Células Cultivadas / Espécies Reativas de Oxigênio / Apoptose / Biologia Celular / Proteínas do Olho / Células Endoteliais da Veia Umbilical Humana / Lipoproteínas LDL Limite: Humans Idioma: Zh Revista: Acta Physiologica Sinica Ano de publicação: 2014 Tipo de documento: Article