FGF8 is Essential for Functionality of Induced Neural Precursor Cell-derived Dopaminergic Neurons
International Journal of Stem Cells
; : 228-234, 2015.
Article
em En
| WPRIM
| ID: wpr-29876
Biblioteca responsável:
WPRO
ABSTRACT
Induced neural precursor cells (iNPCs) are one source of transplantable dopaminergic neurons used in cell therapy for Parkinson's disease. In the present study, we demonstrate that iNPCs can be generated by transducing Brn2, Ascl1, Myt1L and Bcl-xL in a culture supplemented with several mitogens and subsequently can be differentiated to dopaminergic neurons (DA). However, studies have shown that iDA and/or iNPC-derived DA neurons using various conversion protocols have low efficiency. Here, we show that early exposure of FGF8 to fibroblasts efficiently improves differentiation of DA neurons. So our study demonstrates that FGF8 is a critical factor for generation of iNPC-derived DA neurons.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Doença de Parkinson
/
Neurônios Dopaminérgicos
/
Fibroblastos
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Terapia Baseada em Transplante de Células e Tecidos
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Mitógenos
/
Neurônios
Tipo de estudo:
Guideline
Idioma:
En
Revista:
International Journal of Stem Cells
Ano de publicação:
2015
Tipo de documento:
Article