Effect and mechanism of puerarin on high glucose-induced hypo-responses in vascular contraction / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 62-65, 2011.
Article
em Zh
| WPRIM
| ID: wpr-301501
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the effect of puerarin on high glucose-induced decrease in contraction of isolated rat aortic rings, and to elucidate its underlying mechanism.</p><p><b>METHODS</b>The thoracic aortic rings with or without endothelium of male Sprague-Dawley rats were mounted on a bath system. Isometric contractions of aortic rings were measured. The activity of heme oxygenase-1 (HO-1) was also measured.</p><p><b>RESULTS</b>(1) After incubation with 44 mmol/L of glucose (high glucose) for 4 h, the vascular contraction responses to phenylephrine (PE) decreased in an endothelium-dependent manner, when compared with the control group (containing 11 mmol/L of glucose). (2) After coincubation with puerarin ( 10(-10) - 10(-8) mol/L) and high glucose, the decrease in contraction responses to PE of arteries was partly inhibited in a dose-dependent manner. (3) After incubation with puerarin for 4 h, the HO-1 activity of thoracic aorta increased; ZnPP, an inhibitor of HO-1, abrogated the protection effect of puerarin.</p><p><b>CONCLUSION</b>Puerarin could prevent the high glucose-induced decrease in contraction responses to PE in intact aortic rings. The mechanism might be involved in the activation of HO-1.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Aorta Torácica
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Farmacologia
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Fisiologia
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Vasoconstrição
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Vasodilatadores
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Técnicas In Vitro
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Ratos Sprague-Dawley
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Glucose
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Heme Oxigenase (Desciclizante)
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Isoflavonas
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2011
Tipo de documento:
Article