Effects of cyclosporine A on pneumocyte apoptosis with lung ischemia/reperfusion injury in rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 493-496, 2010.
Article
em Zh
| WPRIM
| ID: wpr-301526
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of cyclosporine A (CsA), a powerful inhibitor of mitochondrial permeability transition pore (MPTP), on pneumocyte apoptosis, the release of cytochrome C and the activity of caspase-3 after lung ischemia/reperfusion, and explore the mechanisms.</p><p><b>METHODS</b>Single lung in situ ischemia/reperfusion animal model was used. 30 SD rats were randomly divided into three groups (n = 10) sham (S) group, ischemia/reperfusion (I/R) group and cyclosporine A (CsA) group. Apoptosis of pneumocyte was assessed by TUNEL method, cytochrome C (CytC) in cytoplasm was detected by immunohistochemistry techniques, and the activity of caspase-3 was measured with spectrophotometer.</p><p><b>RESULTS</b>The content of CytC in cytoplasm, the activity of caspase-3, and the value of apoptosis index (AI) in ischemia/reperfusion group were evidently higher than that in S group (P < 0.01). CsA suppressed apoptosis as well as CytC release and caspase-3 activity (P < 0.01).</p><p><b>CONCLUSION</b>CsA can prevent the release of cytochrome C, block the apoptosis of pneumocyte accordingly maybe by closing the MPTP.</p>
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Traumatismo por Reperfusão
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Ciclosporina
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Ratos Sprague-Dawley
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Apoptose
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Biologia Celular
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Citocromos c
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Caspase 3
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Células Epiteliais Alveolares
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2010
Tipo de documento:
Article