The chronic effect of palmitic acid on apoptosis of pancreatic islet beta-cells and the mechanism / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 553-556, 2009.
Article
em Zh
| WPRIM
| ID: wpr-301563
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the chronic effect of palmitic acid (PA) on apoptosis of pancreatic islet beta-cells and the possible mechanism.</p><p><b>METHODS</b>Insulinoma cell line (MIN6 cells) were used in this study. After being incubated in PA (0.1 - 1.6 mml/L) for 24 and 48 hours, MTT method was used to evaluate the livability. After being incubated for 48 h, Hoechst-PI and Annexin-V-FTTC/PI FACS were used to estimate the apoptosis in each group, Western-blotting assay was used to estimate the protein level of p-Akt, Akt, Bax and Bcl-2.</p><p><b>RESULTS</b>Chronic PA dose-dependently (1) decreased the availability and increased the apoptosis of MIN6 cells; (2) decreased the phosphorylation of Akt and Bcl-2, but had no significant effects on Akt and Bax.</p><p><b>CONCLUSION</b>Chronic PA dose-dependently induced apoptosis of MIN6 cells, and this effect was possibly regulated by Akt/Bcl-2.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Fisiologia
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Transdução de Sinais
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Células Cultivadas
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Ilhotas Pancreáticas
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Proteínas Proto-Oncogênicas
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Apoptose
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Estresse Oxidativo
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Ácido Palmítico
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2009
Tipo de documento:
Article